|
KMID : 0377519940190040427
|
|
Chung-Ang Journal of Medicine
1994 Volume.19 No. 4 p.427 ~ p.438
|
|
|
Human Mononuclear Cells Binding to Cultured Human Synovial Fibroblasts by Recombinant Interleukin-1 β
|
|
임병기
장의찬/강수용/강응택/이은우
|
|
|
Abstract
|
|
|
Mononculear cell adhesion to endothelium plays an imporant role in the pathogenesis of chronic inflammatory diseases such as rheumatoid arthritis(RA). In order to define the role and expression of Vascular Cell Adhesion Molecule-1(VCAM-10 and
Intercellular Adhesion Molecule-1(ICAM-1) in arthritic disorders, We studied the expression of those adhesion molecules in synovial tissues of patients with rheumatoid arthritis and the binding capacity of mononuclear cells to cultured normal
synovial
fibroblasts when they were stimulated with recombinant interleukin-1β.
ICAM-1 is expressed on some synovial tissue lining cells and some endothelial cells while VCAM-1 is present more predominently on synovial intimal lining and endothelial cells of venules in RA than ICAM-1.
The amount of VCAM-1 and ICAM-1 was greater in the synovial lining of RA compared with normal synovium.
Both VCAM-1 and ICAM-1 were expressed on cultured synovial fibroblast treated by recombinant interleukin-1 beta(rIL-1β).
After rIL-1βstimulation of cultured synovial fibroblast, mononuclear cell-binding significantly increased, and this mononuclear cell-binding significantly increased, and this increase was blocked by anti-VCAM-1 monoclonal antibody, in the other
hand
anti-ICAM-1 monoclonal antibody did not block the monounclear cell binding.
Therefore the increase in the binding of mononuclear cells to rIL-1βstimulated synovial fibroblast might occur as a result of VCAM-1 dependent factors.
This data suggested that rIL-1βmay regulate the recruitment and retention of mononuclear cell via the up-regulation of VCAM-1 on synovial fibroblast and VCAM-1 pathway may be one of primary important mononuclear cell adhesion to inflamed
synovium.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|